What is the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6?

What is the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6?

• A. C→S→F→A
• B. S→C→A→F
• C. C→S→A→F
• D. S→F→A→C

Answer: (C)

The migration of hemoglobin (Hgb) on agarose or cellulose acetate at pH 8.6 is dictated by the net charge of the different hemoglobin variants under alkaline conditions. Hemoglobins are proteins that can carry a positive or negative charge, which affects their migration during electrophoresis. In this context, at pH 8.6, hemoglobin variants can be expected to migrate based on their specific amino acid compositions and their associated charges. The order of migration for the variants is influenced heavily by their net charge. In the correct order—C (Hb C), S (Hb S), A (Hb A), and F (Hb F)—Hb C has the highest positive charge, so it migrates the farthest in the negative direction. This is followed by Hb S, which is less positively charged compared to Hb C, and thus migrates next. Hb A is neutral in charge and migrates less than Hb C and Hb S. Finally, Hb F, which carries a slightly higher positive charge than Hb A but is less than Hb S, migrates the least of all the mentioned hemoglobins. Understanding the biochemical characteristics of hemoglobin and their behavior in electrophoresis at high pH is key to

The migration of hemoglobin (Hgb) on agarose or cellulose acetate at pH 8.6 is dictated by the net charge of the different hemoglobin variants under alkaline conditions. Hemoglobins are proteins that can carry a positive or negative charge, which affects their migration during electrophoresis.

In this context, at pH 8.6, hemoglobin variants can be expected to migrate based on their specific amino acid compositions and their associated charges. The order of migration for the variants is influenced heavily by their net charge.

In the correct order—C (Hb C), S (Hb S), A (Hb A), and F (Hb F)—Hb C has the highest positive charge, so it migrates the farthest in the negative direction. This is followed by Hb S, which is less positively charged compared to Hb C, and thus migrates next. Hb A is neutral in charge and migrates less than Hb C and Hb S. Finally, Hb F, which carries a slightly higher positive charge than Hb A but is less than Hb S, migrates the least of all the mentioned hemoglobins.

Understanding the biochemical characteristics of hemoglobin and their behavior in electrophoresis at high pH is key to